PATHATRIX is a rapid detection method for pathogens, spoilage bacterium and viruses in food, environmental and bio-threat samples.
The PATHATRIX is a novel patented RIMS (re-circulating immuno-magnetic separation) technology that relies on the use of antibody coated paramagnetic particles to selectively bind and purify the target organism from a comprehensive range of complex matrices. By re-circulating the sample over a "capture phase," comprised of immobilized antibody coated magnetic beads, this increases the sensitivity of the capture. A high volume wash enables the efficient removal of the sample matrices, non-specific microorganisms and PCR inhibitors.
Pathatrix consumables are available in two convenient formats:
- mini-ULTRA / ULTRA (50 ml / 250 ml) for use with the PATHATRIX® ULTRA instruments
- AUTO for use with the new PATHATRIX® AUTO instruments
PATHATRIX® AUTO enables the fully automated capture, concentration and "clean-up" of samples within 15 minutes. The beads are then immediately ready for use with a variety of detection methods: direct plating; PCR; ELISA etc, without any further processing.
Antibody coated beads immobilise onto the "Capture Phase"
Specific capture of target onto the magnet from complex matrices
Captured Bead/Bacteria Complexes are "washed"
Bead / Bacteria complexes are Eluted from Magnet
DETECTION SOLUTIONS
HOW FAST DO YOU WANT RESULTS?
The time to result is dictated by the pre-enrichment incubation of the sample (4-24 hours) and the speed of the end point detection used (40 mins - 48 hours). PATHATRIX® enables results to be available in as little as 5-6 hours from receipt of sample, but this can be tailored to suit the customers' individual needs.
AOAC-RI APPROVED?
Data from internal and external validation studies e.g. AOAC-RI trials have demonstrated that the PATHATRIX® system is significantly more sensitive than many of the current standard methods, at low spike levels 1-10 cfu/25g sample. Full AOAC RI validation reports are available on request together with an extensive bibliography of published data (Technical bulletin).
Ordering Information
For full product details and ordering codes please contact or email us.
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