PATHATRIX is a rapid detection method for pathogens, spoilage bacterium and viruses in food, environmental and bio-threat samples.
The PATHATRIX is a novel patented RIMS (re-circulating immuno-magnetic separation) technology that relies on the use of antibody coated paramagnetic particles to selectively bind and purify the target organism from a comprehensive range of complex matrices. By re-circulating the sample over a "capture phase," comprised of immobilized antibody coated magnetic beads, this increases the sensitivity of the capture. A high volume wash enables the efficient removal of the sample matrices, non-specific microorganisms and PCR inhibitors.
Pathatrix consumables are available in two convenient formats:
- Ultra is unique in that it is the only microbial detection system that can analyze the entire 225 ml + 25 g sample
- mini-Ultra has been specifically designed for environmental and smaller samples found in bio-threat situations, with full sample enclosure.
Once captured and concentrated the beads are ready for use with a variety of detection methods: direct plating; PCR; ELISA etc.
The Pathatrix consumable is loaded onto the instrument with the pre-enriched sample and is circulated for 30 minutes.
The beads are added immediately prior to re-circulation and the beads become immobilized on the capture phase.
The beads are added immediately prior to re-circulation and the beads become immobilized on the capture phase.
During re-circulation, at approx 500 ml per minute, the target microorganism is captured. The high flow rate significantly reduces non-specific binding.
After capture is completed, a high volume (50-100 ml) wash efficiently removes the sample matrices, non-specific miroorganisms, and PCR inhibitors.
Following the wash the removal of the magnet enables the bead/target complexes to be released and concentrated ready for detection.
DETECTION SOLUTIONS
HOW FAST DO YOU WANT RESULTS?
The time to result is dictated by the pre-enrichment incubation of the sample (4-24 hours) and the speed of the end point detection used (40 mins - 48 hours). Pathatrix enables results to be available in as little as 5-6 hours from receipt of sample, but this can be tailored to suit the customers' individual needs.
AOAC-RI APPROVED?
Data from internal and external validation studies e.g. AOAC-RI trials have demonstrated that the PATHATRIX system is significantly more sensitive than many of the current standard methods, at low spike levels 1-10 cfu/25g sample. Full AOAC RI validation reports are available on request together with an extensive bibliography of published data (Technical bulletin).
Ordering Information
For full product details and ordering codes please contact or email us.
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